POLYMERIZATION OF GLUTARALDEHYDE AT FIXATIVE pH
نویسندگان
چکیده
منابع مشابه
Reexamination of the polymerization of pyridoxylated hemoglobin with glutaraldehyde.
Pyridoxylated adult human hemoglobin (HbAo) was prepared using a one molar equivalent of pyridoxal 5-phosphate (PLP) per heme and reduced with either NaCNBH3 or NaBH4. A separate sample was pyridoxylated and passed through a mixed-bed ion exchange column without reduction. All three preparations had a P50 of 29 +/- 2 torr and a cooperativity of n = 2.4 +/- 0.1. These preparations, in both the o...
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Immobilization is an exciting alternative to improve the stability of enzymatic processes. However, part of the applied covalent strategies for immobilization uses specific conditions, generally alkaline pH, where some enzymes are not stable. Here, a new generation of heterofunctional supports with application at neutral pH conditions was proposed. New supports were developed with different bif...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1974
ISSN: 0022-1554,1551-5044
DOI: 10.1177/22.9.911